vsv g encoding plasmid pmd g Search Results


pmd gvsv g  (Addgene inc)
96
Addgene inc pmd gvsv g
Pmd Gvsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pm39383211-71-14-23?v=Addgene+inc
Average 96 stars, based on 1 article reviews
pmd gvsv g - by Bioz Stars, 2026-08
96/100 stars
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vsv g expression plasmid pmd g  (Addgene inc)
98
Addgene inc vsv g expression plasmid pmd g
Vsv G Expression Plasmid Pmd G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pm34246112-173-27-32?v=Addgene+inc
Average 98 stars, based on 1 article reviews
vsv g expression plasmid pmd g - by Bioz Stars, 2026-08
98/100 stars
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type wt vesicular stomatitis virus glycoprotein  (Addgene inc)
96
Addgene inc type wt vesicular stomatitis virus glycoprotein
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Type Wt Vesicular Stomatitis Virus Glycoprotein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/bio_rxiv__2024__06__06__597774-115-2-48?v=Addgene+inc
Average 96 stars, based on 1 article reviews
type wt vesicular stomatitis virus glycoprotein - by Bioz Stars, 2026-08
96/100 stars
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packaging plasmids  (ATCC)
99
ATCC packaging plasmids
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Packaging Plasmids, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pmc03349882-142-23-42?v=ATCC
Average 99 stars, based on 1 article reviews
packaging plasmids - by Bioz Stars, 2026-08
99/100 stars
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vsv-g expression vector (pmd.g  (Polysciences inc)
90
Polysciences inc vsv-g expression vector (pmd.g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Vsv G Expression Vector (Pmd.G, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/bio_rxiv__2023__01__06__522978-220-35-42?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
vsv-g expression vector (pmd.g - by Bioz Stars, 2026-08
90/100 stars
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hiv plasmid vectors prsv rev  (Cell Genesys)
90
Cell Genesys hiv plasmid vectors prsv rev
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Hiv Plasmid Vectors Prsv Rev, supplied by Cell Genesys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pm15385954-63-14-37?v=Cell+Genesys
Average 90 stars, based on 1 article reviews
hiv plasmid vectors prsv rev - by Bioz Stars, 2026-08
90/100 stars
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pmdg vsvg  (Addgene inc)
93
Addgene inc pmdg vsvg
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmdg Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pmc06701927-326-26-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pmdg vsvg - by Bioz Stars, 2026-08
93/100 stars
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pmd.g  (Promega)
90
Promega pmd.g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmd.G, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pmc10191432-204-35-10?v=Promega
Average 90 stars, based on 1 article reviews
pmd.g - by Bioz Stars, 2026-08
90/100 stars
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pmd g vsv g  (Addgene inc)
96
Addgene inc pmd g vsv g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmd G Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pmc06978389-39-20-30?v=Addgene+inc
Average 96 stars, based on 1 article reviews
pmd g vsv g - by Bioz Stars, 2026-08
96/100 stars
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vsv-g glycoprotein expressing plasmid pmdg  (GenScript corporation)
90
GenScript corporation vsv-g glycoprotein expressing plasmid pmdg
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Vsv G Glycoprotein Expressing Plasmid Pmdg, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pmc11112816-184-11-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
vsv-g glycoprotein expressing plasmid pmdg - by Bioz Stars, 2026-08
90/100 stars
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pmd.g  (Cell Genesys)
90
Cell Genesys pmd.g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmd.G, supplied by Cell Genesys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pm16052531-30-18-42?v=Cell+Genesys
Average 90 stars, based on 1 article reviews
pmd.g - by Bioz Stars, 2026-08
90/100 stars
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pmd g  (ATCC)
94
ATCC pmd g
(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular <t>stomatitis</t> virus <t>glycoprotein</t> (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).
Pmd G, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsv+g+encoding+plasmid+pmd+g/pm29611801-52-22-40?v=ATCC
Average 94 stars, based on 1 article reviews
pmd g - by Bioz Stars, 2026-08
94/100 stars
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Image Search Results


(A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).

Journal: bioRxiv

Article Title: A Nanobody Interaction with SARS-CoV-2 Spike Allows the Versatile Targeting of Lentivirus Vectors

doi: 10.1101/2024.06.06.597774

Figure Lengend Snippet: (A) The panel depicts the cellularly expressed Spike protein in blue, with RBD regions as horseshoe shapes. The viral membrane is depicted as pseudotyped with the Tupaia paramyxovirus (TPMV) fusion (F) protein (yellow), associated with a H-Nano chimera of the binding-defective hemaglutinin (H) protein (green) and a nanobody (Nano; red) that binds to the Spike RBD. In this model, binding of the H-Nano protein to Spike activates the TPMV F protein to fuse the viral and cell membranes. (B) The panel depicts an alternative model, in which binding of the viral H-Nano protein to the cellular Spike protein activates Spike to fuse membranes in a TPMV F-independent fashion. (C) β-galactosidase (βGal) reporter lentiviruses were pseudotyped with the binding-defective TPMV H protein plus the TPMV F protein (H+F), or the H-Nano chimeric protein plus F (H-Nano + F), or the H-Nano protein alone (H-Nano). The viruses were used to infect Spike-expressing cells. Infection levels were measured via βGal activities and were normalized to the H-Nano + F levels. Averages and standard deviations are as shown. Note that the H-Nano+F and H-Nano infection levels both were significantly higher than the H+N control (P<0.001). (D) Spike-positive (Spike+) or Spike-negative (Spike-) cells were infected with βGal lentivirus vectors pseudotyped with the wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G), or H-Nano + F, or H-Nano alone. Infection levels for each pseudotyped virus were normalized to infection of the Spike+ cells, and averages and standard deviations are as shown. Note that the difference for the H-Nano virus between Spike+ and Spike-cells was highly significant (P<0.001), while the difference for H-Nano + F was significant (P=0.002).

Article Snippet: The wild type (WT) Vesicular stomatitis virus glycoprotein (VSV G) expression plasmid (pMD.G), the Tupaia paramyxovirus (TPMV) fusion and hemaglutinin expression plasmids (pCG-TPMV-Fd32, pCG-TPMV-Hd32), the SARS-CoV-2 Spike expression plasmid (CoV2-Spike-D614G), the green fluorescent protein expression plasmid (GFP-NoPS), and the lentivirus GagPol packaging plasmid (psPAX2) all were obtained from Addgene, and were the gifts of Simon Davis, Jakob Reiser, Jennifer Doudna, and Didier Trono ( , – ).

Techniques: Membrane, Binding Assay, Expressing, Infection, Control, Virus